NRAS Mutation Detection Kit- Amoy Diagnostics Co.,Ltd.

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NRAS Mutation Detection Kit

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Amoy Diagnostics Co.,Ltd.

Business Type:Manufacturer

Country/Region:China

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Certification:CE
Warranty:1 year

Description

NRAS Mutation Detection Kit

Detection of 16 mutations in NRAS codons 12, 13, 59, 61, 117 and 146

NRAS is a member of the RAS family of small GTPases and plays a central role in the MAPK signaling pathways. NRAS has been implicated in the pathogenesis of several cancers.

NRAS mutations are particularly common in melanoma, hepatocellular carcinoma, myeloid leukemias, and thyroid carcinoma. In total, activating mutations in the NRAS genes occur in 13~25% of cutaneous melanomas, 1~6% of colorectal cancer and 1% of lung cancer,

mainly in exons 2, 3 or 4.

The mutation status of NRAS gene is relevant to drug resistance of non-small cell lung cancer treated with tyrosine kinase inhibitors. As a result, NRAS mutations detection supplies evidence for targeted clinical therapy of tumor patients.

Intended Use

AmoyDx® NRAS Mutation Detection Kit is for Research Use Only (RUO).

Technological Principles

The kit uses novel, patented primers and probes to detect mutations in a real-time PCR assay.

The mutant DNA is amplified accurately by the specific primers, and detected by the novel probes.

Highly Specific primers and probes, and a highly validated procedure based on Taq DNA

polymerase contribute to outstanding assay sensitivity and selectivity.

NRAS mutations detected by the NRAS Kit

Exon	Mutation	Base Change	Cosmic ID	Name
2	G12D	35G>A	564	NRAS-M3
	G12S	34G>A	563	NRAS-M10
2	G13D	38G>A	573	NRAS-M4
2	G13R	37G>C	569	NRAS-M6
	G12C	34G>T	562	NRAS-M7
	G12V	35G＞T	566	NRAS-M9
	G12A	35G>C	565	NRAS-M11
	G13V	38G＞T	574	NRAS-M14
3	A59D	176C＞A	253327	NRAS-M15
3	Q61R	182A>G	584	NRAS-M1
	Q61K	181C>A	580	NRAS-M2
	Q61L	182A>T	583	NRAS-M5
	Q61H	183A>C	586	NRAS-M8
4	K117N	351G＞C	\	NRAS-M16
	K117N	351G＞T	\	NRAS-M17
4	A146T	436G＞A	27174	NRAS-M12

Key Features and Benefits:

• Simple Procedure — only one step required for sample application, results obtained within 90

minutes.

• High sensitivity and accuracy — can detect 1-5% mutant DNA in a background of wild type

genomic DNA.

• ADx-ARMs technology — employs a patented two-step PCR amplification procedure and

novel fluorescent probe design.

• Easy Standardization — any general PCR lab can perform the test, and technicians obtain

reproducible results without specialized training.

• Reliable product quality — Produced in a dedicated GMP plant, with quality management

systems that have passed Chinese CFDA and international ISO13485 standards.

• Sample type: Fresh, frozen or paraffin embedded tissue